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Carl Zeiss
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Olympus
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Oxford Instruments
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Image Search Results
Journal: Cell Reports
Article Title: Re-education of Tumor-Associated Macrophages by CXCR2 Blockade Drives Senescence and Tumor Inhibition in Advanced Prostate Cancer
doi: 10.1016/j.celrep.2019.07.068
Figure Lengend Snippet: Pro-inflammatory Macrophages Induce Growth Arrest and Senescence Enhancement in Pten -Null Tumors (A and B) Bar graph showing fold change in cell proliferation (A) and percentage (B) of SA-b-galactosidase positive human cancer cells exposed to conditioned media from stimulated macrophages. Macrophages were either untreated or stimulated with the following: IFNγ/LPS, IL-4/IL-13, and conditioned media from PC3 cancer cells ± aCXCR2. (C and D) Bar graph showing fold change in cell proliferation (C) and percentage (D) of SA-b-galactosidase positive cells from 22Rv1, LnCaP, and PC3 treated with recombinant TNFα. (E) RT-qPCR analysis of TNFR1 expression in the three human cell lines. (F) Bright-field images from three-dimensional (3D) culture of CRPC patient-derived organoids and cell line-derived organoids (hanging drop) acquired with Zeiss LSM700 confocal laser scanning microscope. (G) RT-qPCR analysis showing the anti-correlation of PTEN and TNFR1 relative expression in human mCRPC patient-derived organoids. Bar chart showing fold change comparison between each gene in reference to a PTEN +/+ control (22Rv1) and Lamin A/C used as a housekeeping gene. Error bars are mean ± SEM (n = 2). ∗ p < 0.05, ∗∗ p = 0.0071 (two-way ANOVA), and ∗∗∗ p < 0.001.
Article Snippet: Macrophages were either untreated or stimulated with the following: IFNγ/LPS, IL-4/IL-13, and conditioned media from PC3 cancer cells ± aCXCR2. (C and D) Bar graph showing fold change in cell proliferation (C) and percentage (D) of SA-b-galactosidase positive cells from 22Rv1, LnCaP, and PC3 treated with recombinant TNFα. (E) RT-qPCR analysis of TNFR1 expression in the three human cell lines. (F) Bright-field images from three-dimensional (3D) culture of CRPC patient-derived organoids and cell line-derived organoids (hanging drop) acquired with
Techniques: Recombinant, Quantitative RT-PCR, Expressing, Derivative Assay, Laser-Scanning Microscopy